The red cells are decreased in number and are normochromic and macrocytic. The peripheral blood smears showed 1 anisopoikilocytosis. A properly prepared blood smear is essential to accurate assessment of cellular morphology. Or, the blood may be examined by an automated machine. Peripheral blood smear showing a trophozoite and a mac. Preparation of smear there are three types of blood smears. In addition, monitoring cytologic changes found in peripheral blood may help determine a patients response to treatment, short and longterm prognoses, and future treatment plan. The best place to examine blood cell morphology is the feathered edge of the blood smear where red cells lie in a single layer, side by side, just barely touching one another but not overlapping. The examination of a peripheral blood smear is one of the most informative exercises a physician can perform. Oct 18, 2016 preparation of smear there are three types of blood smears. This book will be a fullcolor atlas of both benign and neoplastic proliferations in the blood. Blood smear evaluation should be performed as supplement to an automated cbc whether the latter is obtained inhouse or from an outside laboratory.
It takes about 510 minutes to stain a batch of smears. The peripheral blood smear of a patient with hivaids might show anisocytosis, poikilocytosis, and rouleaux formation. Blood films are examined in the investigation of hematological blood disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis. Blood lab first week this is a self propelled powerpoint study atlas of blood cells encountered in examination of the peripheral blood smear. Abnormal monocytes on peripheral blood smears ppt xpowerpoint. Although advances in automated technology have made the examination of a peripheral blood smear by a physician seem less important, the technology is not a completely. Preparation of a peripheral blood film this protocol describes how to prepare a slide film of peripheral blood for microscopic examination. Peripheral blood smear dr ajit nambiar spherocytes causes hereditary spherocytosis immune hemolytic anemia severe burns invitro prolonged storage of blood. A peripheral smear a smear examined by a pathologist is primarily ordered to evaluate blood cell populations when a complete blood count with differential cbcd, performed with an automated blood cell counter, indicates abnormal white blood cells, red blood cells, or platelets. Staining process has begun, no stat slides can be added in the batch. It is simply a thin smear of peripheral blood that contains the cellular components of circulating blood pool.
Section editor lawrence lk leung, md editorinchief hematology section editor disorders of hemostasis and coagulation maureen lyles dambrogio professor of medicine. The wedge smear the cover glass smear the spun smear the are two additional types of blood smear used for specific purposes buffy coat smear thick blood smears for blood parasites 10. Introduction to peripheral blood smear examination oncohema key. Complete exhaustive presentation on peripheral blood smear exam. Synoptic reporting system for peripheral blood smear. Find powerpoint presentations and slides using the power of, find free presentations research about peripheral blood smear evaluation ppt. Hematology exam 1 lecture 2 peripheral blood smears. Peripheral blood smear procedure and interpretation. The test measures the in vitro lysis of rbcs suspended in solutions of decreasing osmolarity. Moderate anisopoikilocytosis occasional oval macrocytes, dacrocytes, and elliptoid cells is seen. During this procedure, a sample of blood is checked for blast cells, number and kinds of white blood cells, number of platelets, and changes in the shape of the blood cells.
A blood filmor peripheral blood smearis a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. The best place to examine blood cell morphology is the. It is found to reduce typing errors, enhance completeness, and improve reporting turnaround time. Complete blood cell count and peripheral blood film, its.
How to perform a proper thick and thin smear for malaria diagnosis duration. One can make smears not only of peripheral blood circulating blood, taken from a vein but also of bone marrow where blood is formed or of inflammatory infiltrate pus. Aqueous solutions of stains are stable only after 36. It is useful to ask the laboratory to generate a wrightsstained peripheral blood smear and examine it. Scroll left and right to see various peripheral blood components. When studying the blood smear slide, pay close attention to nuclear characteristics, staining patterns, and cell diameter remember that erythrocytes are usually 78 m in diameter. A free powerpoint ppt presentation displayed as a flash slide show on id. It is useful to ask the laboratory to generate a wright sstained peripheral blood smear and examine it. State the two samples which may be used to prepare a blood smear. Attention to detail in slide preparation, staining and standard criteria for cell estimates, morphology and differential reporting enhance the value of results provided to the physician. In some ways it is becoming a lost art but it often provides rapid, reliable access to information about a variety of hematologic disorders. Smears should be made within 1 hour of blood collection from edta specimens stored at room.
Hematologyoutlines hematology is the study of blood, bloodforming hematopoietic organs, and neoplasticnonneoplastic blood disorders. Rare apoptotic cells can be seen on normal blood smears. Peripheral smears of chronic leukemias usually show increased numbers of mature cells of a particular lineage i. The examination of blood films stained with wrights stain frequently provides important clues in the. Interpreting peripheral blood smears harrisons principles of. Peripheral smear, peripheral blood film, peripheral blood smear, blood film. Disseminated mycobacterium aviumintracellulare complex mac.
Many hematological diagnoses depend upon this procedure and often a definitive diagnosis can be established from the careful examination of the blood film. The peripheral blood film remains a very useful tool in hematological assessment. The smear is stained with wrights stain and performed after the complete blood count is run. Ppt peripheral blood smear powerpoint presentation. Spherocytes are characterized by membrane loss and less redundancy to withstand. It is then treated with a special stain and studied under a microscope to examine the blood cells. Preparation of peripheral blood smears 1 exercise 10. The phrase blood smear is descriptive of how the preparation is made.
Jun 12, 2016 1 introduction to peripheral blood smear examination. Peripheral blood smear showed schistocytes, spherocytes, smudge cells. This way, each slide may be used to spread the film of the other, and duplicate slides will be of value if something happens to one of them in the. Peripheral blood smear synonyms, peripheral blood smear pronunciation, peripheral blood smear translation, english dictionary definition of peripheral blood smear. A variety of methods are available for preparing and staining blood smears, the most common of which are discussed in this atlas. This synoptic reporting system for peripheral blood smears helps users to draft a complete, accurate, and concise report. Peripheral blood smear definition of peripheral blood. This study module is designed for lsuhsc l2 students enrolled in clinical pathology 201. The examination of the peripheral blood smear is an important basic hematological procedure. View and download powerpoint presentations on peripheral blood smear evaluation ppt.
Hematologyoutlines atlas multiple choice quiz peripheral. Download scientific diagram peripheral blood smear showing a trophozoite and a mac rogametocyte of plasmodium vivax in this patient wrightgiemsa stain. The thickness of the spread when pulling the smear is determined by the 1 angle of the spreader slide the greater the angle, the thicker and shorter the smear, 2 size of the blood drop and 3 speed of spreading. Introduction to peripheral blood smear examination. Jul 15, 2019 a peripheral blood smear examination is a procedure, which involves spreading a drop of blood thinly onto a glass slide. Notice the angle and direction that the spreader slide is pulled, first back towards. Utmedical school at houston, pathology last revision on. Ppt blood smear preparation and staining powerpoint.
Anemia when present is usually normocytic and normochromic. The white blood cells are decreased in number and consist mostly of. Peripheral smear is also known as peripheral blood smear. Trainees and practicing pathologists may benefit from this system, which is freely available on the internet. Peripheral blood smear made from edtaanticoagulated blood. Peripheral blood smear examination macroscopic view.
Blood smear examination powerpoint ppt presentation. It is good practice to prepare two slides for each sample. Blood smear preparation and staining is the property of its rightful owner. Peripheral blood test your knowledge below is a slide at high magnification under an oil immersion lens. Complete blood cell count and peripheral blood film can be truly diagnostic for a disease condition.
Peripheral blood smear 1 peripheral blood smear 2 introduction. It is often done as part of or along with a complete blood count cbc. The white blood cells are decreased in number and consist mostly of blasts 41% as reported on the differential. Tutorial blood cell morphology a clinical pathology 201. Sometimes macrocytic anemia can be seen even in the absence of zidovudine therapy.
Peripheral blood film or peripheral blood smear or peripheral smear examination forms an important and basic lab investigation for diagnosing a number of hematological blood disorders. With the advent of hematological automated machines, complete and highly accurate hematological data and parameters can be obtained within a few minutes. It involves multiple disciplines, including pathology, physiology, internal medicine, pediatrics, and laboratory medicine. Geisinger medical laboratoriesgeisinger proven diagnostics. A peripheral blood smear examination is a procedure, which involves spreading a drop of blood thinly onto a glass slide.
This picture illustrates the proper procedure for making blood smears. A blood smear is a blood test that gives information about the number and shape of blood cells. Peripheral blood smear spherocytes osmotic fragility a laboratory test used in the diagnosis of hs, is sensitive but not specific. The test provides information on the number and shape of these. The wedge smear is the most convenient and commonly used technique for making pbs. Dec 17, 2010 how to perform a proper thick and thin smear for malaria diagnosis duration. Start studying hematology exam 1 lecture 2 peripheral blood smears incomplete. Find powerpoint presentations and slides using the power of, find free presentations research about abnormal monocytes on peripheral blood smears ppt. You should look for other peripheral blood smear abnormalities pertinent to a diagnosis of leukemia. Hematologic manifestations of hivaids clinical gate. On the peripheral blood smear, apoptotic lymphocytes show nuclear shrinkage, pyknosis, fragmentation, and blebs. Hematology exam 1 lecture 2 peripheral blood smears incomplete study.
It is intended as a reference for blood cell and bone marrow morphology. This page includes the following topics and synonyms. View and download powerpoint presentations on abnormal monocytes on peripheral blood smears ppt. View normal values, symptoms, peripheral blood smear test results, procedure to conduct. A blood smear is a blood test used to look for abnormalities in blood cells. Hematologyoutlines hematology is the study of blood, blood forming hematopoietic organs, and neoplasticnonneoplastic blood disorders. Use the oil immersion lens to identify each cell type. The blood smear should occupy the central portion of the slide and should not touch the edges. Red blood cells rbcs, erythrocytes light red, biconcave discs 7. The presentation of illustrative cells in this module is by no means a comprehensive study of blood cells. Peripheral blood smear introduction a properly prepared blood smear is essential to accurate assessment of cellular morphology the wedge smear is the most convenient. Peripheral blood smear test about, preparation, test. The three main blood cells that the test focuses on are. Learn vocabulary, terms, and more with flashcards, games, and other study tools.
Author david s rosenthal, md professor emeritus harvard medical school. Introduction to peripheral blood smear examination clinical. Ppt peripheral blood smear powerpoint presentation free. The cbc with differential presently includes a blood smear that is examined by the medical technologist and is available for the physician. Peripheral blood smear and bone marrow morphology in hivaids. Ppt blood smear examination powerpoint presentation, free. Preparation of peripheral blood smears blood viscosity and angle. What is a peripheral smear, what will it indicate in the. Prepares an acceptable blood smear for routine hematological studies using proper blood drop size. It remains a frontline diagnostic in unraveling mysteries behind cryptic symptoms and signs in primary and secondary haemopathies. Second, appearance of the organism in peripheral blood, especially in cats, can be. The elements seen, altogether, are not representative of normal peripheral blood, but are portrayed for learning and identification purposes. Peripheral blood smear test purpose, procedure, results.
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